In the initial colonies of Wistar rats in Strasbourg, 30% of the animals showed spontaneous SWDs. Irritation of the nasal cavity characterized by hyperplasia of the olfactory epithelium was seen in a time-related fashion for all methyl bromide-treated groups. weight. Results. These results show that metaplasia produced in the rat olfactory epithelium was a threshold response upon chronic inhalation exposure to methyl bromide and that a high control incidence of metaplasia is noted in aged rats. In the Wistar Han rat study, 31 males presented with 34 total palpable masses, while 46 females presented with 53 total palpable masses. Rats are usually supplied feed free choice and they eat 10-30 g a day (5 g/100 g body weight/day). Once the stereotaxic coordinates (AP=−0.8, DV=4.2, and L=±1.5) were determined for the lateral ventricles of the brain, two holes were drilled on the skull and the injection via cannula was inserted in the ventricles. Observations were made twice daily for mortality and changes in general appearance or behaviour. WAG/Rij are fully inbred rats, which means that they are homozygous for all autosomal genes. Gotoh et al. Weight cycling (We) induced by ad-liband restrict­ ed high fat (HF) feeding has been shown to reduce final body weight but not body fat percent in female Wistar rats. Both Wistar and SD Rat can be used as models of HF diet-induced obesity, although most of the metabolic effects caused by HF diet were more pronounced, or earlier detected, in Wistar Rat. The animals were fasted for 12 h with free access to water before the experiment. Researchers collected additional data that may prove helpful in determining whether the Wistar Han rat is the right fit for your study. Rats are usually supplied feed free choice and they eat 10-30 g a day (5 g/100 g body weight/day). Interim sacrifice of 10 mice/sex/treatment level was performed after 6 and 15 months of exposure. A high oral lethal dose (LD50) of 3,707 mg/kg was observed in the acute toxicity test. 2. Moreover, one moderate lesion of the nasal mucosa was also observed in a control animal at the 24-month sacrifice interval. The data include food consumption, organ weight, complete blood count, coagulation, cause of death (for unscheduled decedents), and serum chemistry profiles. Coffee is one of the most commonly consumed beverages in the worldwide and is assumed to have protective effects against metabolic syndrome. ICV-STZ impairs brain glucose metabolism and reduces ATP/ADP ratio, probably through creation of an imbalance between energy production and consumption. In females, the range is from 0.67 g to animals between 170-200 g body weight, up to 0.8 g ani- A strain, in reference to rodents, is a group in which all members are, as nearly as possible, genetically identical. In addition, all but one of the lesions in the 3-ppm exposure level group were described as slight or very slight. Sprague . In rats, this is accomplished through inbreeding. Macroscopic and microscopic examinations were performed on all animals. Wistar rats given 5g/kg of Remifemin® granulate for 26 weeks did not show any organ or chemical toxicity [108] nor was toxicity observed in dogs given 400mg/kg/day of black cohosh for 26 weeks [109]. Finally, the samples were embedded in Epon/Araldite (Fluka): a mixture of 475 ml Epon (order nr: 45345), 245 ml DDSA (order nr: 45346), and 280 ml MNA (order nr: 45347). Transferred to Charles River UK in 1996. Weight cycling (WC) induced by ad-lib and restricted high fat (HF) feeding has been shown to reduce final body weight but not body fat percent in female Wistar rats. Naylor, in Encyclopedia of Basic Epilepsy Research, 2009. Ten adult Wistar rats weighing between 103 and 180 g were allocated into two Groups of A and B after 3 weeks leave of acclimatization. Feed may be pelleted or powdered. By Alternatively, picrotoxin (30 μM) is used to block GABAA inhibitory currents, either with DNQX (10 μM) with 0 Mg2+ in the ACSF or with APV (50 μM) to isolate NMDA and non-NMDA EPSCs, respectively. Young 3-months-old (m.o.) Taconic received breeding stock from RCC in 1998. Wistar albino rats. A post-test experimental study was … The present study was aimed at investigating the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high fructose diet. 7 (0.04908) time since birth: wet weight - Wistar rat: Baye2002a: tWw_C41: see Fig. exposed F344 rats to methyl bromide concentrations of 0, 4, 20, and 100 ppm on a 6 h/day, 5 day/week basis for 104 weeks. Their heads were kept motionless in a stereotaxic surgery device in order to do a longitude cut on the posterior part of the head to access the skull. In acute and 14-day studies, administration of the FOSSENCE TM to Wistar rats did not cause any mortality or clinical signs and changes in body weights, feed consumption, and gross pathology at the doses of 2000, 5000, and 9000 mg/kg body weight. Whole cell patch clamp recordings from somata of visualized neurons are made using borosilicate microelectrodes. The Effect of Imperata cylindrica on Body Weight of Wistar Rats After 28 Day Treatment . Jpn. The Wistar-Kyoto (WKY) rat is an established depression model characterized by elevated anxiety- and depression-like behavior across a variety of tests. The spontaneously diabetic rat, now termed the "BB" rat, was first recognized in 1974 at the Bio Breeding Laboratories of Canada Ltd. (Ottawa) in an outbred colony of rats derived from a Wistar strain. Wistar rats encompass a number of rat substrains that have been derived from a common lineage over the past 50+ years. The dry powder of SGD was immersed in distilled water overnight. The Wistar Han rat has been a top choice for many leading pharmaceutical, chemical and contract research organizations due to the many advantages it offers, such as smaller body size and, Envigo Teklad’s standard Global diets (consisting of 16% protein, 4 %fat). The lesions were characterized as very slight, slight, or moderate. Wistar IGS Rat Details. SGD (0.22 g/mL) was administered orally to the rats of group A at a dose of 1 mL/100 g body weight. Although other Wistar substrains are commonly used in specialized carcinogenesis and safety studies, these strains offer neither a substantial history of use in chronic carcinogenicity bioassays nor a significant publicly available database of historical control pathology and clinical pathology data. The sections were observed in an electron microscope EM400 (Philips (FEI), Eindhoven, The Netherlands) and recorded on a Morada digital camera (OlympusSIS, Münster, Germany). Twenty adult albino wistar rats with average weight of 130-150 gms were randomly assigned into two groups. Also, at this concentration, body weights, especially for females, were lower than the control group from week 4 and throughout the remainder of the study, and decreased absolute brain weight was noted for females. 2. weight compared with the absolute heart weight of females in normal adult albino rats. Cancer Res.91, 368–374, April 2000 368 Dose- and Sex-related Carcinogenesis by N-Bis(2-hydroxypropyl)nitrosamine in Wistar Rats Eduardo L. T. Moreira,1 João L. V. de Camargo,2, 4 Maria A. M. Rodrigues,2 Luis F. Barbisan3 and Daisy M. de Favero Salvadori2 1Departamento de Patologia e Clínicas, Escola de Medicina Veterinária, UFBA, 40170-110, BA, Brasil, ABSTRACT: The effect of Neen seed oil on body and organ weights of male Wister rats were investigated were invested. The Wistar Han rat has been a top choice for many leading pharmaceutical, chemical and contract research organizations due to the many advantages it offers, such as smaller body size and high rates of survival. Our researchers recently completed a two-year study of 130 male and 130 female Envigo RccHan®:WIST (Wistar Han®) rats and compared them to other outbred rats (e.g., Sprague-Dawley®, Fischer 344). The stereotactic frame was self-made (commercially available frames can be purchased from Kopf Instruments, Tujunga, California). Nomenclature: ArcCrl:WI: Product code: WI: Strain Description: Outbred: Coat Colour: Albino: MHC Haplotype: Outbred: Females are good foster mothers. Indeed, both data sets demonstrate that transmission of SWDs is inherited (Marescaux et al., 1992a; Peeters et al., 1990b, 1992). We examined the metabolic conse­ quences of this type of WC. The cerebellum of each rat was removed and-weighed before fixing in 10% formol saline for routine histological procedures. The extract administration was in two phases. The optimal animal model can help expedite the research process and improve study outcomes, while at the same time reduce cost and support the principles of the 3Rs. Twenty adult albino wistar rats with average weight of 130-150 gms were randomly assigned into two groups. Low background lesion incidence is particularly helpful in carcinogenicity studies, as it can lead to more sensitivity in the evaluation of test compounds. Biochemical, haematological and histological Analysis were realized at the end of each study. A limitation of this study is that we evaluated only male Wistar rats. In sub-acute toxicity study, female rats were divided into 4 groups of 5 animals each. Three quantitative trait loci (QTLs) were identified on chromosomes 4, 7, and 8, which appear to control different variables of SWD (e.g., frequency, amplitude, severity). The rats were weighed with a Mettler balance and the weights recorded at the beginning and on the 28 th day of the study. The spontaneously diabetic rat, now termed the "BB" rat, was first recognized in 1974 at the Bio Breeding Laboratories of Canada Ltd. (Ottawa) in an outbred colony of rats derived from a Wistar strain. The rat is the most commonly used rodent species for toxicology studies. Group A served as the control. In the second experiment, male Wistar rats, 60 days old, were divided into three groups (n = 8): control (C) given free access to a control chow; (S) receiving the control chow and drinking 30% sucrose ad libitum and (HC) fed a high-carbohydrate diet ad libitum. Wistar rats (220–250 g body weight) were purchased from the laboratory animal center of Heilongjiang University of Chinese Medicine (Harbin, China) and divided into two groups (group A, drug group for dosed rat plasma, n = 7, and group B, control group for blank rat plasma, n = 7) and kept in a breeding room. In the three-fold high-iodine supplement group, goiter resumed partly and oxidation damage abated, but the activity of antioxidation enzymes of the thyroid (such as GSH-PX and SOD) and the ability of integrated antioxidation significantly decreased. Wistar rats at iodine deficient baseline were divided into two groups and fed on water of normal and three-fold high iodine level, respectively, for 24 weeks. Zhongyan Shan, ... Yaxin Lai, in Comprehensive Handbook of Iodine, 2009. The Wistar rat, Wistar Hannover (Wistar HAN) rat, and Wistar Unilever (WU) rat are outbred rat strains, while the Wistar Kyoto and Wistar Furth rats are inbred strains. For males weighing over 450 grams, the weight of the heart can reach up to 1 gram. The body weights of the Wistar rats decreased significantly (p < 0.05) in the treated Groups B and C compared with the controls. After having taken the biopsies the rats were killed under anaesthesia by neck dislocation. Strain) aged between 12 and 14 weeks and with a body weight of 150 – 180g. In conclusion, cardiac structure increase is associated with body weight gain during Wistar rat … rats had the largest daily fluid intakes and drinking responses to hypertonic and dehydrational stimuli, suggesting that they have In female Wistar Han rats, the body weights were even lower – about 30% lower than the CD female weights at 104 weeks. We use cookies to help provide and enhance our service and tailor content and ads. Ping Wang, ... Xijun Wang, in Serum Pharmacochemistry of Traditional Chinese Medicine, 2017. The results provided insights on how Wistar Han provided numerous advantages, such as smaller body size, higher rates of survival and lower tumor/lesion incidence, as compared to CRL:CD®(SD) (CD) rat models that were the subjects of a previous two-year study. For instance, male Wistar Han body weights were at least 20% lower than the weights of CD rats through the 104 week mark. An equal dose of distilled water was orally administered to the rats of group B. Wistar rats obtained from the Central Animal Facility (University of Bern) were treated according to Swiss animal protection laws. The outbred Wistar and Wistar HAN strains have been used widely in Europe for preclinical safety assessments, including 2-year carcinogenicity bioassays. In female Wistar Han rats, the body weights were even lower – about 30% lower than the CD female weights at 104 weeks. The rats were allowed to feed on commercially prepared r at pellet and provided with fresh water ad libitum. Here, we share the results of the study. This also supports the principles of the 3Rs – replacement, reduction and refinement. Sprague . Oxyclozanide is an effective anthelmintic and has shown good properties in other ways including anti-adenovirus, anti-biofilm, antifungal, and antibacterial activity. A statistically significant increase was found between controls and the low-dose group (3 ppm) at the end of the exposure period (29 months). The Effect of Imperata cylindrica on Body Weight of Wistar Rats After 28 Day Treatment . B6C3F1 mice were exposed via inhalation (whole body) to concentrations of 0, 10, 33, or 100 ppm methyl bromide on a 6 h/day, 5 days/week basis for 2 years (NTP, 1992). Obes Res. Interim necropsies of 20 rats per gender were performed at one, three and six months, respectively, with the final necropsy of 70 rats of each gender at 24 months. (1997), using four serial KA episodes of status epilepticus in Sprague-Dawley rats, found no deficits in spatial learning retention following the status. The rats were acclimatised for 14-day period to the environment with free access to food and water. Body weight is a key component of chronic toxicity and carcinogenicity studies due to the lengthy duration of these studies. Rats, Wistar Rats, Inbred WKY Rats, Inbred SHR Rats, Inbred Strains Rats, Inbred WF Rats, Mutant Strains Rats, Gunn Rats, Inbred BB Animals, Newborn Euphorbiaceae Rats, Inbred F344 Diseases 9 Disease Models, Animal Body Weight Diabetes Mellitus, Experimental Hypertension Reperfusion Injury Epilepsy, Reflex Rodent Diseases Carcinoma 256, Walker Tissue Adhesions By contrast, outbred populations are used when identical genotypesare unnecessary or a population with genetic variation is required, and these … The study confirmed that the Wistar Han rat is a reliable model for toxicity and carcinogenicity studies. We use an Impedence Meter for determining the stage of estrus in rats prior to breeding. Table 2 Examples regarding, the increase of blood glucose (mg/dl) following the anesthesia (xylayine-ketamine) in White Wistar rats Blood glucose (mg/dl) Nr. The optimal animal model can help expedite the research process and improve study outcomes, while at the same time reduce cost and support the principles of the 3Rs. DREMEL 400-4/80 (Migros) is a cordless electric drill we used to make 2-mm-diameter holes into the rat skull. All individuals display SWDs on their EEG. Neither the observed weight of the liver, nor its weight relative to the body weight (without the growth) was correlated with the tumor weight. : 13365 ) was administered orally to the rats were acclimatised for 14-day period the! Wound and neuralagia post-dose ) did not reveal any gross pathological abnormalities a avoidance! 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