The Wistar strain maintained since last century has not been typed genetically so far1. injections of more than 200 ul? The original stock was obtained from the Wistar Institute (Philadelphia, PA). Although metabolic changes have been described in this strain, nothing is … For Verapamil, Wistar Han and Sprague-Dawley strains do not show differences between male and female rats. How can I do these two dilutions so that I can perform an i.p. It has the distinct honor of being the first rat stock developed to serve as a model animal. Yes sir I got all answers. DNA samples are suitable for PCR, Southern blot analysis, and DNA library construction. Very commonly used outbred rat. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction. Thank you so much for your exlanation.. it is really useful to differentiate two strains by visual appearance.. Another ilportant thing is: the three strains present also behavioral differences that you have to take into account depending on the task you are planing to use. Lab rat strain genealogy. The Wistar audiogenic rat (WAR) is an animal model of tonic-clonic epileptic seizures, developed after genetic selection by sister × brother inbreeding of Wistar rats susceptible to sound stimuli. The Sprague-Dawley and Wistar stocks tend to be more docile while the Fischer 344 and Long-Evans and other hooded rats are more difficult to handle. G), and ultraline TiO 2 (P25, Degussa, FRG) and were subsequently kept in clean air for 6 mo. Fisher 344 (F344) and SD rat strains generally have the highest tumor incidences, exceeding that in Wistar rats. Spontaneous clonic convulsions reported. It has thus been concluded that Wistar rats are the best experimental model for silicosis, as their pulmonary reaction is more characteristic than that of the other two strains. Rederived by GlaxoWellcome from Han Wistar stock supplied by BRL. Han ® is a registered trademark of Envigo. It has the distinct honor of being the first rat strain developed to serve as a model animal. There are many differences which I can't reproduce easily. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). All reagents needed for isolatation, analysis, and storage of RNA  are included, making kit of an excellent value. Kitano M., Hatano H., and Shisa H. (1992) Strain difference of susceptibility to 4-nitroquinoline 1-oxide- induced tongue carcinoma in rats. I'm not entirely sure which strain of rat we are working with, but I've narrowed it to Lewis/Wistar rats. Kim U., Clifton K. H., and Furth J. A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. Rat strain Tox TK Total SD 80 42 122 HW 80 60 140 TK: 6 timepoints (0.5 mL/timepoint), 2 occasions Extra 18 TK HW rats Solution: microsampling 28 day study + Total number of rats on study (8-10 wk old rats) + Han Wistar rats are smaller, lower blood draw per rat Please contact Zyagen technical support for the availability of theses products. I hope I have answered your question and it may help you in your research work. Purified RNA can be used in a number of downstream applications including real-time PCR, reverse…, Sprague Dawley Strain, Rat Tissue Total RNA, Match Set of Mouse Tissue RNA, DNA & Protein, Match Set of Rat Tissue RN, DNA & Protein, CD1 Mouse Brain Regions Frozen Tissue Sections, Mouse CD1 Brain Sagittal & Horizontal Frozen Tissue Sections, Mouse CD1 Brain & Spinal Cord Floating Frozen Tissue Sections, Mouse CD1 Brain Coronal Frozen Tissue Sections, Mouse CD1 Spinal Cord Frozen Tissue Sections, CD1 Mouse Placenta Frozen Tissue Sections, C57 Mouse Brain Regions Frozen Tissue Sections, Mouse C57 Sagittal & Horizontal Frozen Sections, C57 Mouse Placenta Frozen Tissue Sections, BALBC Mouse Brain Regions Frozen Tissue Sections, Mouse Balb/C Brain Region Sagittal & Horizontal Frozen Tissue Sections, Mouse Balb/C Brain Region Coronal Frozen Tissue Sections, Sprague Dawley Rat Frozen Tissue Sections, SD Rat Brain Regions Frozen Tissue Sections, Rat SD Brain Sagittal & Horizontal Frozen Tissue Sections, Rat SD Brain Coronal Frozen Tissue Sections, Rat SD Spinal Cord Frozen Tissue Sections, Rat SD Brain and Spinal Cord Floating Frozen Tissue Sections, Wistar Rat Brain Coronal Frozen Tissue Sections, Wistar Rat Brain Sagittal & Horizontal Frozen Tissue Sections, CD1 Mouse Brain Regions Paraffin Tissue Sections, Mouse CD1 Brain Sagittal & Horizontal Paraffin Sections, Mouse CD1 Brain Coronal Paraffin Sections, CD1 Mouse Embryo Sagittal Paraffin Tissue Sections, CD1 Mouse Embryo Coronal Paraffin Tissue Sections, CD1 Mouse Placenta Paraffin Tissue Sections, CD1 Mouse Mammary Paraffin Tissue Sections, C57 Mouse Brain Regions Paraffin Tissue Sections, Mouse C57 Brain Sagittal & Horizontal Paraffin Tissue Sections, Mouse C57 Brain Coronal Paraffin Tissue Sections, C571 Mouse Embryo Paraffin Tissue Sections, C57 Mouse Placenta Paraffin Tissue Sections, C57 Mouse Mammary Paraffin Tissue Sections, BALBC Mouse Brain Regions Paraffin Tissue Sections, Mouse BALBC Brain Region Sagittal & Horizontal Paraffin Tissue Sections, Mouse BALBC Brain Region Coronal Paraffin Tissue Sections, Sprague Dawley Rat Paraffin Tissue Sections, SD Rat Brain Regions Paraffin Tissue Sections, Rat SD Brain Sagittal & Horizontal Paraffin Tissue Sections, Rat SD Brain Coronal Paraffin Tissue Sections, Rat SD Spinal Cord Paraffin Tissue Sections, Wistar Rat Brain Coronal Paraffin Tissue Sections, Wistar Rat Brain Sagittal & Horizontal Paraffin Tissue Sections, Wistar Rat Tissue Paraffin Tissue Sections. Le rat Lou/C, issu de la souche Wistar, reste maigre tout au long de sa vie. Which raw materials can we use for making pellet for Laboratory rats? As noted above, WKY rats were originally bred as a control strain for SHR rats, with both lines being derived from a common Wistar strain (Okamoto and Aoki, 1963). This has markedly diluted the original strain. The extracted protein is provided in vials at a concentration of 5mg/ml and ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction. J. Natl. 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