blood smear technique pdf

0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (3)Tj ET BT 98.762 709.936 TD 0 Tc 0 Tw (5. 0000002691 00000 n What makes cytology unique is that it can tell us the result of the diseases while the )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (Smears should be air-dried, and then dipped into 100% methanol. H��WɎ��:��2"r=Z�� G�h�DY\�1��U�9�M�i�`*_WV.��a ��8��%I\KiQ�kJqa)k-iyz\~ҏZ|��)��~�>/��0��-�J��q��?��T�O��^_��\�o~�.,߾�nY��Q�}��~��]8��8ۋmL��R]*�5�Z��ˋW��~��ç��O��aː/��|��O�\~��mJ��Û�߽��Ǐo��?�T�Hj�ZVI�W�\Ԯq�H��ߖ:��K ��֖��e��7��ut��/i� 1�RႊqipG`��R'u��,��|.0V��"�N ;�,fێtZ�-�XŊ��' a#X�jƸe[8�.�\��.�n��RkK�y��+8��z�ˀS��F�u�VuC8��U u��,_��;L��j��_F��^�W#α{5��f �5"a\[��>3��I9��|�G�8��(��:0��w\��2!��P�r8�fš͘��n�\��K�L�-&[� \^}{ M�\l��@�t�!��y��>��E `. How to cite this article: Mehta V, Vasanth V, Balachandran C. Pap smear. Take one drop of venous or capillary blood. A smooth action is required, with the edge)Tj ET BT 116.043 126.243 TD (of the spreader held against the slide. This will yield a nice, even smear. 0000077459 00000 n Blood can also be taken from an EDTA sample. Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. Finger Prick or . )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (First prepare the buffer. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. 0000134854 00000 n Mix 9.5 gm with distilled water to make 1000 mL. 0000078886 00000 n )Tj ET BT 98.762 301.207 TD (3. 0000111872 00000 n Smears made)Tj ET BT 98.762 566.653 TD (in the field in hot and dry climates often are of very poor quality, probably because they)Tj ET BT 98.762 550.573 TD (dry too rapidly. Although this is a higher pH than normally used to stain blood cells, the)Tj ET BT 116.043 407.289 TD (parasites will stain darker and be more visible under the microscope. 5. It must be differentiated from the non-pathogenic microfilaria of Dipetalonema reconditum (“Dipet” for short). The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. %%EOF 0000080309 00000 n It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. How to Make a Peripheral Blood Smear. View lab report.pdf from CHS 123 at Aquinas University of Legazpi. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (There is no need to cover-ship the slides. 0000199779 00000 n 2. )Tj ET BT 98.762 216.245 TD (10. Slides can be stored while drying in a small plastic slide)Tj ET BT 116.043 359.528 TD (box \(holds 25 slides\). The most common technique of blood smear preparation is called the “wedge or push” technique. 0000318262 00000 n 6. WEDGE BLOOD SMEAR • Specimen: Peripheral blood smear made from EDTA- anticoagulated blood. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (A high-quality Giemsa should be used. 2014-‐09-‐01 Blood Page 1 of 5 Lab: Blood Smear and RBC Count Aim: Learn to count cells, 3. A poor slide is a torment. One slide serves as the blood smear slide and the other as the spreader slide. 0000088426 00000 n In the field we use blue plastic slide boxes that hold 25 slides. 0000134021 00000 n the examination of a blood smear when such protocols indicate that it is necessary. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (A single smear can be made per slide \(smear running the length of the slide\) or two)Tj ET BT 116.043 428.65 TD (\(or even three\) smears can share a slide, with the smears running the width of the)Tj ET BT 116.043 412.809 TD (slide. Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. )Tj ET endstream endobj 23 0 obj 2879 endobj 21 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F1 6 0 R /F2 7 0 R >> /ProcSet 2 0 R >> /Contents 22 0 R >> endobj 6 0 obj << /Type /Font /Subtype /TrueType /Name /F1 /BaseFont /Times-Roman /Encoding /MacRomanEncoding >> endobj 7 0 obj << /Type /Font /Subtype /TrueType /Name /F2 /BaseFont /Times-Bold /Encoding /MacRomanEncoding >> endobj 10 0 obj << /Type /FontDescriptor /FontName /ArialMT /Flags 32800 /FontBBox [ -255 -208 1021 896 ] /MissingWidth 278 /StemV 93 /StemH 93 /ItalicAngle 0 /CapHeight 718 /XHeight 531 /Ascent 896 /Descent -208 /Leading 42 /MaxWidth 1021 /AvgWidth 551 /Style << /Panose <0508020B0600000000000000> >> >> endobj 11 0 obj << /Type /Font /Subtype /TrueType /Name /F3 /BaseFont /ArialMT /FirstChar 0 /LastChar 255 /Widths [ 0 750 750 750 750 750 750 750 0 278 750 750 750 0 750 750 750 750 750 750 750 750 750 750 750 750 750 750 750 0 750 750 278 278 355 556 556 889 667 191 333 333 389 584 278 333 278 278 556 556 556 556 556 556 556 556 556 556 278 278 584 584 584 556 1015 667 667 722 722 667 611 778 722 278 500 667 556 833 722 778 667 778 722 667 611 722 667 944 667 667 611 278 278 278 469 556 333 556 556 500 556 556 278 556 556 222 222 500 222 833 556 556 556 556 333 500 278 556 500 722 500 500 500 334 260 334 584 750 667 667 722 667 722 778 722 556 556 556 556 556 556 500 556 556 556 556 278 278 278 278 556 556 556 556 556 556 556 556 556 556 556 400 556 556 556 350 537 611 737 737 1000 333 333 549 1000 778 713 549 549 549 556 576 494 713 823 549 274 370 365 768 889 611 611 333 584 549 556 549 612 556 556 1000 278 667 667 778 1000 944 556 1000 333 333 222 222 549 494 500 667 167 556 333 333 500 500 556 278 222 333 1000 667 667 667 667 667 278 278 278 278 778 778 750 778 722 722 722 278 333 333 333 333 333 333 333 333 333 333 ] /Encoding /MacRomanEncoding /FontDescriptor 10 0 R >> endobj 2 0 obj [ /PDF /Text /ImageC /ImageI ] endobj 5 0 obj << /Kids [4 0 R 12 0 R 15 0 R 18 0 R 21 0 R ] /Count 5 /Type /Pages /MediaBox [ 0 0 612 792 ] >> endobj 1 0 obj << /Creator (Microsoft Word 98) /CreationDate (D:20050725111313) /Subject () /Title () /Author (jschall) /Producer (Acrobat PDFWriter 4.05 for Power Macintosh) /Keywords () >> endobj 3 0 obj << /Pages 5 0 R /Type /Catalog /DefaultGray 24 0 R /DefaultRGB 25 0 R >> endobj 24 0 obj [/CalGray << /WhitePoint [0.9505 1 1.0891 ] /Gamma 1.8008 >> ] endobj 25 0 obj [/CalRGB << /WhitePoint [0.9505 1 1.0891 ] /Gamma [1.8008 1.8008 1.8008 ] /Matrix [0.3954 0.2208 0.0411 0.4022 0.6391 0.1576 0.1528 0.1405 0.8903 ] >> ] endobj xref 0 26 0000000000 65535 f 0000025678 00000 n 0000025517 00000 n 0000025870 00000 n 0000003649 00000 n 0000025564 00000 n 0000023776 00000 n 0000023889 00000 n 0000000017 00000 n 0000003629 00000 n 0000024001 00000 n 0000024306 00000 n 0000013140 00000 n 0000003790 00000 n 0000013119 00000 n 0000016843 00000 n 0000013271 00000 n 0000016822 00000 n 0000020547 00000 n 0000016975 00000 n 0000020526 00000 n 0000023645 00000 n 0000020690 00000 n 0000023624 00000 n 0000025959 00000 n 0000026039 00000 n trailer << /Size 26 /Root 3 0 R /Info 1 0 R /ID [] >> startxref 26208 %%EOF. 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